Anti-CD11b / Mac-1 alpha Monoclonal Antibody (Clone:MEM-174)

CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18) ; expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.

Product Specifications

Product Name Alternative

ITGAM||CD11B||CR3A

Gene Name

ITGAM

Gene ID

3684

UniProt

P11215

Host

Mouse

Reactivity

Human

Immunogen

Human granulocytes

Target Antigen

CD11b

Clonality

Monoclonal

Clone

MEM-174

Applications

FACS, IP

Purification

Purified by protein-A affinity chromatography

Format

Purified

Storage Conditions

Store at 2-8°C. Do not freeze.

Applications Notes

Flow Cytometry Recommended dilution:1 μg/mL Positive control: Peripheral Blood Lymphocytes Immunoprecipitation Positive control: GranulocytesCD11b/CD18 transfectants Application note: Immunoprecipitation with the antibody MEM-174 was successfuly performed using immuno-affinity sorbents (minicolumns) . The final immunosorbent should contain 1-5 mg of antibody/1 ml of sorbent gel (e.g. agarose beads) It is recommended to stimulate positive control material by LPS 1-2 days before to achieve better expression of target antigen) .