Anti-Cytokeratin 18 Monoclonal Antibody (Clone:DC-10)

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.Cytokeratin 18 belongs to type I family (acidic cytokeratins) .

Product Specifications

Product Name Alternative

KRT18||CYK18||PIG46

Gene Name

KRT18

Gene ID

3875

UniProt

P05783

Host

Mouse

Reactivity

Human

Immunogen

Human breast carcinoma cell line PMC-42.

Target Antigen

Cytokeratin 18

Clonality

Monoclonal

Clone

DC-10

Applications

ELISA, IHC, FACS, WB

Purification

Purified by protein-A affinity chromatography

Format

Purified

Storage Conditions

Store at 2-8°C. Do not freeze.

Applications Notes

Western blotting: Recommended dilution: 1-2 μg/ml. Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml; positive tissue: colon, skin. Immunocytochemistry: positive control: MCF-7 human breast adenocarcinoma cell line, HeLa human cervix carcinoma cell line. ELISA: Positive control: MCF-7 human breast adenocarcinoma cell line; the antibody DC-10 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04. Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.