Anti-IL-33 (human), mAb (IL33305B)

Recognizes human IL-33. Does not cross-react with mouse IL-33.Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30kDa human IL33 is converted by CASP1 to a 18kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1 (MAPK3) /ERK2 (MAPK1), p38 (MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis

Product Specifications

Product Name Alternative

Interleukin-33; IL-1F11; NF-HEV

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human IL-33.

Clone

IL33305B

Applications

Functional Assay, ELISA, IHC

Components

Concentration 1mg/mL Formulation: Liquid. 0.2 μm-filtered solution in PBS, pH 7.4. Contains no preservatives

Storage Conditions

Stable for at least 1 year after receipt when stored at -20°C.

Applications Notes

ELISA: (direct or indirect: 1:2'000-1:5'000) Functional Application (Blocking) Immunohistochemistry (paraffin sections (1:50-1:200) ) Immunoprecipitation Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.