Biotinylated Anti-His Tag Antibody (DM1) ; Rabbit mAb
The rabbit immune system generates antibody diversity and optimizes affinity. Anti-His Tag Antibody (DM1) ; Rabbit mAb is specific to six histidine tags placed at C-terminal and internal regions of fusion proteins. This antibody can greatly improve the effectiveness of several different kinds ofimmunoassays; helping researchers identify; detect; and purify polyhistidine fusion proteinsin bacteria; insect cells; and mammalian cells.
Product Specifications
Product Name Alternative
His tag
Gene Name
His Tag
Host
Rabbit
Reactivity
Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl- terminus.
Target Antigen
His Tag
Clonality
Monoclonal
Clone
DM1
Applications
ELISA; WB
Purification
Purified from cell culture supernatant by affinity chromatography
Components
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Storage Conditions
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing) . Lyophilized proteins are shipped at ambient temperature.
Applications Notes
WB 1:1000
Isotype
IgG
Frequently Asked Questions
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