Biotinylated Anti-His Tag Antibody (DM1) ; Rabbit mAb

The rabbit immune system generates antibody diversity and optimizes affinity. Anti-His Tag Antibody (DM1) ; Rabbit mAb is specific to six histidine tags placed at C-terminal and internal regions of fusion proteins. This antibody can greatly improve the effectiveness of several different kinds ofimmunoassays; helping researchers identify; detect; and purify polyhistidine fusion proteinsin bacteria; insect cells; and mammalian cells.

Product Specifications

Product Name Alternative

His tag

Gene Name

His Tag

Host

Rabbit

Reactivity

Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl- terminus.

Target Antigen

His Tag

Clonality

Monoclonal

Clone

DM1

Applications

ELISA; WB

Purification

Purified from cell culture supernatant by affinity chromatography

Components

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

Storage Conditions

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing) . Lyophilized proteins are shipped at ambient temperature.