Anti-Rotavirus (Clone: RV6-26)

Reactivity Species : Rotavirus-Virus Expression Host : HEK-293 Endotoxin Level :≤ 1.0 EU/mg as determined by the LAL method. Specificity : RV6-26 activity is directed against a quaternary epitope on VP6. This human monoclonal antibody RV6-26 binds at the viral RNA release pore in the DLP and is able to neutralize RV by inhibiting transcription. Structural data suggest steric hindrance inhibits the transcriptional pore. Background : Rotaviruses (RV) are double-stranded, non-enveloped, icosahedral RNA viruses in the Reoviridae family that cause severe dehydrating diarrhea in infants and children. RV particles are composed of concentric viral protein (VP) layers. The triple-layered particle has an inner capsid layer (VP2), an intermediate capsid layer (VP6), and an outer capsid layer (VP7, VP4) . The transcriptionally active double-layered particle (DLP) consists of VP2 and VP6. VP6 is the most antigenic RV protein in humans. The antibody VH146 gene segment is the site of primary interaction with VP65.

Product Specifications

Host

Human

Immunogen

Human Mab RV6-26 was sequenced from RV-specific B cells isolated from the blood of healthy adult donors or RV-infected infants or adults

Clonality

Monoclonal

Clone

RV6-26

Applications

ELISA, FACS

Purification

>=95% monomer by analytical SEC

Components

>= 5.0 mg/mL Formulation : This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Storage Conditions

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at >= -70°C. Avoid Repeated Freeze Thaw Cycles.