Phospho-MARCKS (Ser167/170) (Clone: C9) rabbit mAb FITC conjugate

MARCKS (myristoylated alanine-rich C kinase substrate) is a major PKC substrate expressed in all eukaryotic cells (1,2) . It binds to and cross-links actin filaments to serve as a bridge between Ca2+/calmodulin and PKC signaling and attenuates phosphatidylinositol 4,5-bisphosphate plasma membrane signaling (3) . MARCKS is involved with cell mobility, phagocytosis, membrane traffic, cell adhesion, and mitogenesis. Ser159, 163, 167 and 170 of MARCKS are phosphorylated by PKC in response to cell groeth and cellular stress (4) . MARCKs phosphorylation is believe to induce its tranlocation from plasma membrane to cytoplasm.

Product Specifications

Product Name Alternative

Myristoylated alanine-rich C-kinase substrate, Protein kinase C substrate, PKCSL, 80 kDa protein light chain, MACS, PRKCSL

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Ser167/170 of human phospho MARCKS

Clonality

Monoclonal

Clone

MARCKSS167170-C9

Applications

FACS

Components

1X PBS, 0.09% NaN3, 0.2% BSA

Storage Conditions

Store at 2-8°C. Avoid repeated freeze and thaw cycles.

Applications Notes

For flow cytometric staining, the suggested use of this reagent is 5 μL per million cells or 5 μL per 100 μL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.