Phospho-SAPK/JNK (Thr183/Tyr185) (Clone: A11) rabbit mAb

The SAPK/JNK pathway initiates apoptosis upon exposure to radiation, UV exposure, heat shock, oxidative stress, and other stressors. Upon exposure to environmental stress, the SAPK/JNK signaling pathway sequentially activates the proteins MEKK1, SEK1, SAPK, and c-Jun. Upstream activators of the SAPK/JNK cascade include ceramide, small GTP-binding proteins such as Rac1 and Cdc42Hs, Ask1, and caspases. MKK7 is also a major and direct SAPK/JNK activator in the TNF alpha or environmental stress signaling pathways, where its kinase activity directly phosphorylates SAPK/JNK. This relationship between MKK7 and SAPK appears to be evolutionarily conserved, as it is preserved in their Drosophila homologues, Hep and DJNK, respectively.

Product Specifications

Product Name Alternative

Mitogen-activated protein kinase 8, MAPK8, Stress-activated protein kinase 1c, SAPK1c, c-Jun N-terminal kinase 1, JNK1, PRKM8

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Thr183/Tyr185 of human phospho SAPK/JNK

Clonality

Monoclonal

Clone

SAPKT183Y185-A11

Applications

FACS

Format

Purified

Components

1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA

Storage Conditions

Store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

1μg/mL - 0.001μg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information. (0.5mg/ml, more than 200 western blots)