Phospho-PKCa (Thr497) (Clone: F1) rabbit mAb

PKC alpha is a calcium-dependent isozyme of the PKC family that phosphorylates serine/threonine residues in apoptosis and cellular proliferation and differentiation pathways, including the MAPK cascade. PKC alpha directly phosphorylated Raf-1, inducing survival genes. An increase in PKC alpha is associated with multi-drug resistance in cancer cell lines, and increased expression in breast cancers is noted as causing a particularly malignant phenotype. Thus PKC alpha has been the target of novel cancer therapeutics, with some promising developments in microRNA inhibitors. PKC alpha is itself phosphorylated by mTOR. PKC alpha also plays an important role in water regulator and solute absorption in the cell, where it regulates aquaporin 2 by initiating AQP2 ubiquination and lysosomal degradation.

Product Specifications

Product Name Alternative

Protein kinase C alpha type, PKC-alpha, PRKCA, PKCA, PRKACA

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Thr497 of human phospho PKC alpha

Clonality

Monoclonal

Clone

PKCaT497-F1

Applications

FACS, WB

Format

Purified

Components

1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA

Storage Conditions

Store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

1μg/mL - 0.001μg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information. (0.5mg/ml, more than 200 western blots)