Phospho-Stat5 (Tyr694) (Clone: G11) rabbit mAb

Stat5 activation occurs in response to many ligands including prolactin, IL-2, growth hormone, and GM-CSF. Tyr694 phosphorylation is obligatory activation of Stat5 (1,2), and is mediated by Src upon erythropoietin stimulation (3) . Phospho Stat5 is constitutively active in some leukemic cell types (4), and phospho Stat5 is found in some endothelial cells when treated with IL-3, suggesting its involvement in cell motility and angiogenesis (5) . Stat5 has been shown to be encoded by two separate genes, Stat5a and Stat5b, which share over 90% amino acid sequence identity. In different cell types, Stat5a and Stat5b are independently regulated and activated. For example, interferon treatment predominantly activates Stat5a in U937 cells and Stat5b in HeLa cells (6) .

Product Specifications

Product Name Alternative

Signal transducer and activator of transcription 5A, STAT5A, Signal transducer and activator of transcription 5B, STAT5B

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Tyr694 of human phospho Stat5

Clonality

Monoclonal

Clone

Stat5Y694-G11

Applications

FACS, WB

Format

Purified

Components

1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA

Storage Conditions

Store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

1μg/mL - 0.001μg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information. (0.5mg/ml, more than 200 western blots)