Phospho-MKK3 (S189) /MKK6 (S207) (Clone: D3) rabbit mAb APC conjugate

MKK3 and MKK6 are closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5) . Phospho MKK3 and phospho MKK6 both phosphorylate and activate p38. p38 phosphorylation dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5) . Phospho MKK3 and phospho MKK6 activation occurs through phosphorylation at S189 and T222 on MKK3 (2) and S207 and T211 on MKK6 (4,5) .

Product Specifications

Product Name Alternative

Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MAPK/ERK kinase 3, Stress-activated protein kinase kinase 2, SAPK kinase 2, SAPKK2, MAP2K3, MEK3, PRKMK3, SKK2

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Ser189 of human phospho MKK3 and Ser207 of human phospho MKK6.

Clonality

Monoclonal

Clone

MKK3S189MKK6S207-D3

Applications

FACS

Format

Conjugated

Components

1X PBS, 0.09% NaN3, 0.2% BSA

Storage Conditions

Store at 2-8°C. Avoid repeated freeze and thaw cycles.

Applications Notes

For flow cytometric staining, the suggested use of this reagent is 5 μL per million cells or 5 μL per 100 μL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.