Phospho-Histone H2A.X (Ser139) (Clone: 1E4) rabbit mAb SureLight 488 conjugate

Histone H2AX is a variant of the nucleosome core histone H2A and is phosphorylated at Ser139 in response to DNA damage. Histone H2AX phosphorylation is considered a specific reporter of double-strand DNA breaks. The protein is also referred to as gH2AX when phosphorylated at Ser139. H2AX phosphorylation is especially strong in response to double-strand breaks formed during apoptosis. However, physiological phosphorylation of Histone H2AX occurs when double-strand DNA breaks are formed during meiosis and V (D) J recombination. A549 and DU145 cell lines have been found to have higher expression levels of phosphorylated Histone H2AX compared to Jurkat, MCF-7, or HL-60 cell lines.

Product Specifications

Product Name Alternative

H2AFX, H2AX, ?H2AX, gamma-H2AX

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Ser139 of human phospho histone H2A.X.

Clonality

Monoclonal

Clone

HisH2AXS139-1E4

Applications

FACS

Format

Conjugated

Components

1X PBS, 0.09% NaN3, 0.2% BSA

Storage Conditions

Store at 2-8°C. Avoid repeated freeze and thaw cycles.

Applications Notes

For flow cytometric staining, the suggested use of this reagent is 5 μL per million cells or 5 μL per 100 μL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.