Phospho-PLCg1 (Tyr783) (Clone: C4) rabbit mAb APC conjugate

The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositolphosphate to inositol triphosphate and diacylglycerol. In response to extracellular stimuli such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate diacylglycerols (DAGs) and water-soluble phosphorylated derivatives, such as inositol 1,4,5-triphosphate (IP3) . Within the PLC family, PLCg is the only member that contains SH2 and SH3 domains, necessary for phospho PLCg activation. Phospho PLCg, upon activation, can interact with receptor tyrosine kinases.

Product Specifications

Product Name Alternative

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, PLC-148, Phosphoinositide phospholipase C-gamma-1, Phospholipase C-II, Phospholipase C-gamma-1, PLC-gamma-1, PLC1

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Tyr783 of human phospho PLCg1.

Clonality

Monoclonal

Clone

PLCG1Y783-C4

Applications

FACS

Format

Conjugated

Components

1X PBS, 0.09% NaN3, 0.2% BSA

Storage Conditions

Store at 2-8°C. Avoid repeated freeze and thaw cycles.

Applications Notes

For flow cytometric staining, the suggested use of this reagent is 5 μL per million cells or 5 μL per 100 μL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.