Phospho-NFkB p65 (Ser536) (Clone: C8) rabbit mAb

The nuclear factor kB (NFkB) /Rel family of transcription factors play a pivotal role in inflammatory and immune responses (1,2) . NF-kappa-B is present in almost all cell types and is involved in many biological processes including immunity, inflammation, cell growth and differentiation, apoptosis, and tumorigenesis. NFkB is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFkB1/p105, NFkB1/p50, REL and NFkB2/p52. The dimers bind at kB sites in the target gene DNA. Individual dimers have distinct preferences for different kB sites and can act as either transcriptional activators or repressors. NFkB Ser536 phosphorylation stimulates Lys310 acetylation and interaction of phospho NFkB with CBP. Acetylated/phospho NFkB induces enhanced transcriptional activity.

Product Specifications

Product Name Alternative

Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, NFKB3

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Ser536 of human phospho NFkB.

Clonality

Monoclonal

Clone

NFkBS536-C8

Applications

Format

Purified

Components

1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA

Storage Conditions

Store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

1μg/mL - 0.001μg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information. (0.5mg/ml, more than 200 western blots)