Phospho-Stat1 (Tyr701) (Clone: 3E6) rabbit mAb

Stat1 mediates the cellular response to IFN alpha, IFNB, and IFNg for the regulation of cell growth and the defense against viral and immune challenges. The Jak-Stat pathway plays a central role in the IFNg response, where Stat1 phosphorylation on Tyr701 causes homodimerization through its SH2 domain, translocation to the nucleus, and binding to gamma-activated sequence (GAS) elements. Early in the activation sequence, Stat1 is also phosphorylated at Ser727 through a mechanism involving PI3 kinase and Akt. Stat1 has been found to correlate with increased resistance to chemotherapeutic drugs. However, Stat1 activation of the immune system helps suppress tumor growth, and multiple melanomas and squamous-cell carcinomas have been known to downregulate Stat1 expression to evade immune surveillance.

Product Specifications

Product Name Alternative

Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Tyr701 of human phospho Stat1

Clonality

Monoclonal

Clone

Stat1Y701-3E6

Applications

FACS

Format

Purified

Components

1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA

Storage Conditions

Store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

1μg/mL - 0.001μg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information. (0.5mg/ml)