Phospho-Chk2 (Thr68) (Clone: D12) rabbit mAb

Checkpoint kinase 2 (Chk2) plays a major role in the checkpoint response to DNA damage. Chk2 is initially inactive in its monomeric, unphosphorylated form. Phosphorylation at Thr68 induces homodimerization, initiating autophosphorylation within the kinase loop at Ser516 and phorphorylation events within the auto-inhibitory loop at Thr383 and Thr387. After these phosphorylations, active dimers and monomers can then phosphorylate substrates such as Cdc25C and BRCA1. In humans, Chk2 genetic deletion and missense variants have been found to be associated with increased risk of breast and colon cancer. Constitutively phosphorylated Chk2 at Thr68 has been found in many human cancer cell lines, especially ones with mutations in p53.

Product Specifications

Product Name Alternative

Serine/threonine-protein kinase Chk2, Cds1 homolog, Hucds2, CHEK2, RAD53

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Thr68 of human phospho Chk2

Clonality

Monoclonal

Clone

Chk2T68-D12

Applications

FACS, WB

Format

Purified

Components

1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA

Storage Conditions

Store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

1μg/mL - 0.001μg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information. (0.5mg/ml)