Phospho-CrkL (Tyr207) (Clone: G4) rabbit mAb PE conjugate

CrkL (v-Crk sarcoma virus CT10 oncogene-like protein) is an adaptor protein composed of one Src Homology 2 (SH2) and two Src Homology 3 (SH3) domains separated by flexible linker sequences that act as building blocks to assemble multiprotein complexes (1) . The Crk adaptor proteins (Crk and CrkL) constitute an integral part of a network of essential signal transduction pathways in humans and other organisms that act as major convergence points in tyrosine kinase signaling. CRKL is required for the normal development of multiple tissues that rely on fibroblast growth factor 8 (FGF8) . Phosphorylation of Crk on Tyr 221 or CrkL on Tyr 207 causes intramolecular binding of the linker region to the SH2 domain, sequestering the SH2 and SH3N and preventing them from binding target proteins (2,3) . Mounting evidence indicates that dysregulation of Crk proteins is associated with human diseases, including cancer and susceptibility to pathogen infections.

Product Specifications

Product Name Alternative

Crk-like protein; CRKL; v-crk sarcoma virus CT10 oncogene homolog (avian) -like

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Tyr207 of human phospho CrkL

Clonality

Monoclonal

Clone

CrkLY207-G4

Applications

FACS

Format

Conjugated

Components

1X PBS, 0.09% NaN3, 0.2% BSA

Storage Conditions

Store at 2-8°C. Avoid repeated freeze and thaw cycles.

Applications Notes

For flow cytometric staining, the suggested use of this reagent is 5 μL per million cells or 5 μL per 100 μL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.