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Anti-Villin (GI-Mucosal & Urogenital Brush Border Marker) Recombinant Mouse Monoclonal Antibody (Clone:rVIL1/1325)

Selected Variant

12-1264-20

Size: 20 µg

Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

Product Specifications

Product Name Alternative

VIL1; Villin-1; Villin1

Gene Name

VIL1

Gene ID

7429

UniProt

P09327

Host

Mouse

Reactivity

Human

Immunogen

Recombinant fragment of human Villin (aa 179-311) (exact sequence is proprietary)

Clonality

Monoclonal

Clone

RVIL1/1325

Applications

IHC

Purification

Protein A/G

Format

Purified

Components

200μg/mL of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Immunohistochemistry (Formalin-fixed) (1-2μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)

Isotype

Mouse IgG1, kappa

Price

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