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Anti-GFAP (Astrocyte & Neural Stem Cell Marker) Recombinant Mouse Monoclonal Antibody (Clone:rASTRO/789)

This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP) . It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Product Specifications

Product Name Alternative

Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)

Gene Name

GFAP

Gene ID

2670

UniProt

P14136

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant full-length human GFAP protein

Clonality

Monoclonal

Clone

RASTRO/789

Applications

FACS, WB, IHC

Purification

Protein A/G

Format

Purified

Components

200μg/mL of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Flow Cytometry (1-2μg/million cells) ; Western Blot (1-2μg/mL) ; Immunohistochemistry (Formalin-fixed) (0.25-0.5μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)

Isotype

Mouse IgG1, kappa

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