Anti-Ferritin, Light Chain (FTL) (Microglia Marker) Recombinant Rabbit Monoclonal Antibody (Clone:FTL/2338R)

Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III) . Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.

Product Specifications

Product Name Alternative

Ferritin L chain; Ferritin L subunit; Ferritin light chain; Ferritin light polypeptide; FTL; LFTD; NBIA3

Gene Name

FTL

Gene ID

2512

UniProt

P02792

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant fragment of human FTL protein (around aa 38-165) (exact sequence is proprietary)

Clonality

Monoclonal

Clone

FTL/2338R

Applications

WB, IHC

Purification

Protein A/G

Format

Purified

Components

200μg/mL of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Western Blot (1-2μg/mL) ; Immunohistochemistry (Formalin-fixed) (1-2μg/mL for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)