Anti-Cytokeratin, Multi (Epithelial Marker) Recombinant Mouse Monoclonal Antibody (Clone:rKRT/457)

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI<5.7) and basic (pI>6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4) ; 58kDa (CK5) ; 56kDa (CK6) ; 52kDa (CK8) ; 56.5kDa (CK10) ; 53kDa (CK13) and 45kDa (CK18) . This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Product Specifications

Gene Name

Not Known

Gene ID

3851 (CK4) ; 3852 (CK5) ; 3853 (CK6A) ; 3854 (CK6B)

UniProt

P02538 (CK6A) ; P04259 (CK6B) ; P13647 (CK5) ; P19013 (CK4) ; P48668 (CK6C

Host

Mouse

Reactivity

Human

Immunogen

Keratin-enriched preparation from cultured human epithelial cells

Clonality

Monoclonal

Clone

RKRT/457

Applications

IHC

Purification

Protein A/G

Format

Purified

Components

200μg/mL of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Immunohistochemistry (Formalin-fixed) (1-2μg/mL for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)