Anti-CD43 (T-Cell Marker) Recombinant Mouse Monoclonal Antibody (Clone:rSPN/839)

It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43 [Workshop IV]. Epitope of MAb Bra7G is clearly different from that of MAb rSPN/839, called "b" as opposed to "a" for rSPN/839. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

Product Specifications

Product Name Alternative

Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, Sialophorin, SPN

Gene Name

SPN

Gene ID

6693

UniProt

P16150

Host

Mouse

Reactivity

Human

Immunogen

Human full-length recombinant CD43 protein

Clonality

Monoclonal

Clone

RSPN/839

Applications

FACS, WB, IF, IHC

Purification

Protein A/G

Format

Purified

Components

200μg/mL of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Flow Cytometry (1-2μg/million cells) ; , Western Blot (1-2μg/mL) ; , Immunofluorescence (1-2μg/mL) ; , Immunohistochemistry (Formalin-fixed) (1-2μg/mL for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)