Anti-AIF1 / Iba1 (Microglia Marker) Recombinant Mouse Monoclonal Antibody (Clone:rAIF1/1909)

AIF1 is a cytoplasmic, calcium-binding protein that is thoμght to play a role in macrophage activation and function. AIF1, containing two EF domains, is induced by cytokines and Interferons. In an unstimulated state, AIF1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding AIF1 resides in the tumor necrosis factor (TNF) cluster of genes, located in the region represented by the human major histocompatibility complex (MHC) .

Product Specifications

Product Name Alternative

Allograft inflammatory factor 1 (AIF1) ; balloon angioplasty responsive transcription (BART1) ; IBA1; Interferon responsive transcript 1; Ionized calcium-binding adapter molecule 1; IRT1; Microglia response factor (MRF1)

Gene Name

AIF1

Gene ID

199

UniProt

P55008

Host

Mouse

Reactivity

Human

Immunogen

Purified recombinant human AIF1 protein (around aa 1-146) (exact sequence is proprietary)

Clonality

Monoclonal

Clone

RAIF1/1909

Applications

IHC

Purification

Protein A/G

Format

Purified

Components

200μg/mL of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Immunohistochemistry (Formalin-fixed) (1-2μg/mL for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)