Recombinant Rabbit Monoclonal Antibody to CD79a (Clone : IGA/1688R)

A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B cell Ag receptor. CD79a first appears at pre B cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines. Anti-CD79a is generally used to complement anti-CD20 especially for mature B-cell lymphomas after treatment with Rituximab (anti-CD20) . This antibody will stain many of the same lymphomas as anti-CD20, but also is more likely to stain B-lymphoblastic lymphoma/leukemia than is anti-CD20. Anti-CD79a also stains more cases of plasma cell myeloma and occasionally some types of endothelial cells as well.

Product Specifications

Product Name Alternative

B lymphocyte-specific MB1 protein, B-cell antigen receptor complex-associated protein alpha chain, CD79a molecule immunoglobulin associated alpha, Ig-alpha, IGA, IgM-alpha, Immunoglobulin-associated alpha, Ly54, MB-1 membrane glycoprotein, Membrane-bound immunoglobulin-associated protein, Surface I

Gene Name

CD79A

Gene ID

973

UniProt

P11912

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to aa 202-216 (GTYQDVGSLNIADVQ) of human CD79a protein.

Target Antigen

CD79a

Clonality

Monoclonal

Clone

IGA/1688R

Applications

WB, IF, FACSIHC

Purification

Purified Ab with BSA and Azide at 200ug/ml

Format

Purified

Components

200ug/mL of Ab purified Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Western Blot (1-2μg/mL) ; Immunofluorescence (1-2μg/mL) ; Flow Cytometry (1-2μg/million cells) ; Immunohistochemistry (Formalin-fixed) (1-2μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)