Recombinant Rabbit Monoclonal Antibody to CD43 (T-Cell Marker) (Clone : SPN/1766R)

It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

Product Specifications

Product Name Alternative

Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, Sialophorin, SPN

Gene Name

SPN

Gene ID

6693

UniProt

P16150

Reactivity

Human

Immunogen

Recombinant full-length human SPN protein

Target Antigen

CD43 (T-Cell Marker)

Clonality

Monoclonal

Clone

SPN/1766R

Applications

WB, FACS, IF, IHC

Purification

Purified Ab with BSA and Azide at 200ug/ml

Format

Purified

Components

200ug/mL of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Western Blot (1-2μg/mL) ; Flow Cytometry (1-2μg/million cells) ; Immunofluorescence (1-2μg/mL) ; Immunohistochemistry (Formalin-fixed) (1-2μg/mL for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)