Recombinant Rabbit Monoclonal Antibody to CD45RB (B-Cell Marker) (Clone : PTPRC/1783R)

CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out) . The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin.

Product Specifications

Product Name Alternative

B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Gene Name

PTPRC

Gene ID

5788

UniProt

P08575

Reactivity

Human

Immunogen

Human B-lymphocytes

Target Antigen

CD45RB (B-Cell Marker)

Clonality

Monoclonal

Clone

PTPRC/1783R

Applications

FACS, IF, WB, IHC

Purification

Purified Ab with BSA and Azide at 200ug/ml

Format

Purified

Components

200ug/mL of Ab purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (1-2μg/million cells) ; Immunofluorescence (1-2μg/mL) ; Western Blot (1-2μg/mL) ; , Immunohistochemistry (Formalin-fixed) (1-2μg/mL for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes)