Recombinant Rabbit Monoclonal Antibody to CD31 / PECAM-1 (Endothelial Cell Marker) (Clone : C31/1395R)

CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

Product Specifications

Product Name Alternative

EndoCAM, PECA1, Platelet Endothelial Cell Adhesion Molecule 1, GPIIA'

Gene Name

PECAM1

Gene ID

5175

UniProt

P16284

Reactivity

Human

Immunogen

Human recombinant full-length CD31 protein

Target Antigen

CD31 / PECAM-1 (Endothelial Cell Marker)

Clonality

Monoclonal

Clone

C31/1395R

Applications

WB, FACS, IHC

Purification

Purified Ab with BSA and Azide at 200ug/ml

Format

Purified

Components

200ug/mL of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Western Blot (1-2μg/mL) ; Flow Cytometry (1-2μg/million cells) ; Immunohistochemistry (Formalin-fixed) (1-2μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)