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Recombinant Rabbit Monoclonal Antibody to Cytokeratin 7 (Glandular and Transitional Epithelial Marker) (Clone : KRT7/1499R)

Selected Variant

12-1028-100

Size: 100 µg

It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-) .

Product Specifications

Product Name Alternative

CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7

Gene Name

KRT7

Gene ID

3855

UniProt

P08729

Reactivity

Human

Immunogen

Recombinant full-length human KRT7 protein

Target Antigen

Cytokeratin 7 (Glandular and Transitional Epithelial Marker)

Clonality

Monoclonal

Clone

KRT7/1499R

Applications

FACS, WB, IHC

Purification

Purified Ab with BSA and Azide at 200ug/ml

Format

Purified

Components

200ug/mL of Ab purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (1-2μg/million cells) ; Western Blot (1-2μg/mL) ; Immunohistochemistry (Formalin-fixed) (1-2μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95&degC followed by cooling at RT for 20 minutes)

Isotype

Rabbit IgG

Price

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