Recombinant Mouse Monoclonal Antibody to Ep-CAM / CD326 (Epithelial Marker) (Clone : rVU-1D9)

This antibody reacts with the first EGF repeat in the extracellular domain of Ep-CAM. It is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Product Specifications

Product Name Alternative

Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TROP1, Tumor-associated Calcium Signal Transducer 1 (TACSTD1), ECS-1, Epidermal Surface Antigen 1, ESA1, FLOT2, F

Gene Name

EPCAM

Gene ID

4072

UniProt

P16422

Reactivity

Human

Immunogen

Small cell lung carcinoma cells

Target Antigen

Ep-CAM / CD326 (Epithelial Marker)

Clonality

Monoclonal

Clone

RVU-1D9

Applications

FACS, IHC

Purification

Purified Ab with BSA and Azide at 200ug/ml

Format

Purified

Components

200ug/mL of recombinant MAb purified Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry (0.5-1μg/million cells) ; Immunohistochemistry (Formalin-fixed) (1-2μg/mL for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)