Polyclonal Antibody to ASAP3

ASAP3 belongs to Arf-specific GTPase-activating proteins which regulate Arfs by stimulating their intrinsic GTP hydrolysis. This protein contains a catalytic core of a PH (Pleckstrin Homology) domain, a GAP motif, and two ANK (Ankyrin) repeats. ASAP3 also associates with focal adhesions and with cytoplasmic membranes at the circular dorsal ruffles, where the actin skeleton is remodeled. Depletion of ASAP3 by RNA interference suppresses cell migration in wound healing.

Product Specifications

Product Name Alternative

ASAP3||DDEFL1||UPLC1

Gene Name

ASAP3

Gene ID

55616

UniProt

Q8TDY4

Host

Rabbit

Reactivity

Human

Immunogen

A partial length recombinant ASAP3 protein (amino acids 550-800) was used as the immunogen for this antibody.

Target Antigen

ASAP3

Clonality

Polyclonal

Applications

Purification

Protein A Chromatography

Format

Purified

Components

25 μg in 50 μl/100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Western blot analysis: 2-4 μg/mL This antibody detects ~43 kDa band. The size corresponds to Isoform 2 (Uniprot ID-Q8TDY4-2) of ASAP3, the right size is ~99 kDa.