Polyclonal Antibody to Spi-B
Spi-B belongs to the ETS family transcription containing a conserved ETS domain which specifically binds to a purine-rich DNA sequence called the PU-box (5'-GAGGAA-3') . Spi-B has two known isoforms (28 and 18 kDa) localized in nucleus and cytoplasm. Spi-B contains two transactivation domains (TAD) at the N- terminal end which may be required for transcriptional activation of the target genes. Spi-B is involved in development of plasmacytoid dendritic cells (natural interferon producing cells) and B-cell development. Spi-B acts as a lymphoid specific enhancer. Spi-B is highly expressed in plasmacytoid dendritic cells (pDCs), B-cells and stem cell populations of the liver.
Product Specifications
Product Name Alternative
SPIB
Gene Name
SPIB
Gene ID
6689
UniProt
Q01892
Host
Rabbit
Reactivity
Human
Immunogen
A partial length recombinant Spi-B protein (amino acids 50-260) was used as the immunogen for this antibody.
Target Antigen
Spi-B
Clonality
Polyclonal
Applications
IHC, WB
Purification
Protein A Chromatography
Format
Purified
Components
25 μg in 50 μl/100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Western blot analysis: 2-4 μg/mL, Immunohistochemical analysis: 5 μg/mL
Isotype
Rabbit IgG
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