Polyclonal Antibody to Histone H3.3
Histones are major chromatin proteins involved in nucleosome assembly. Their major function is to compact long DNA strands inside the nucleus and chromatin regulation. Histone H3.3 is a replication independent variant of Histone H3 which is generally expressed in quiescent or terminally differentiated cells. Modifications in Histone H3.3 are associated with transcriptional activation and acts as an epigenetic mark of transcriptionally active chromatin. Histone H3.3 is a 15 kDa protein which is expressed throughout the cell cycle independently of DNA synthesis in all eukaryotic genomes.
Product Specifications
Product Name Alternative
H3F3A||H3.3A||H3F3||PP781
Gene Name
H3F3A
Gene ID
3020
UniProt
P84243
Host
Rabbit
Reactivity
Mouse, Human
Immunogen
Full length recombinant Histone H3.3 protein was used as the immunogen for this antibody.
Target Antigen
Histone H3.3
Clonality
Polyclonal
Applications
IHC, WB
Purification
Protein A Chromatography
Format
Purified
Components
25 μg in 50 μl/100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Western blot analysis: 1-2 μg/mL, Immunohistochemical analysis: 5 μg/mL
Isotype
Rabbit IgG
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