Polyclonal Antibody to ICAD
ICAD/DFF45 is an inhibitor of the caspase-activated DNase (CAD, DFF40) . DNA fragmentation factor (DFF) consists of two subunits, a 40 kDa caspase-activated nuclease (DFF40) and a 45 kDa inhibitor (DFF45) . In proliferating cells CAD or DFF40 forms a complex with ICAD or DFF45. When cells undergo apoptosis, caspase 3 cleaves ICAD at two sites to dissociate the CAD:ICAD complex, allowing CAD to cleave chromosomal DNA. Thus DEF induces the fragmentation of DNA which is a hallmark of apoptosis. ICAD is expressed in brain, blood, skin, spleen, adipose tissue. It has 2 isoforms produced by alternative splicing.
Product Specifications
Product Name Alternative
DFFA||DFF1||DFF45||H13
Gene Name
DFFA
Gene ID
1676
UniProt
O00273
Host
Rabbit
Reactivity
Mouse
Immunogen
A partial length recombinant ICAD protein (amino acids 111-331) was used as the immunogen for this antibody.
Target Antigen
ICAD
Clonality
Polyclonal
Applications
WB
Purification
Protein A Chromatography
Format
Purified
Components
25 μg in 50 μl/100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Western blot analysis: 4-6μg/mL
Isotype
Rabbit IgG
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