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Polyclonal Antibody to SARS-CoV-2 nucleocapsid Protein Biotin Conjugated

The structural nucleocapsid (N) protein of nCoV/SARS-CoV-2/COVID-19 is a predicted 46 kDa phosphoprotein having 419 amino acid residues. A short Serine rich stretch and a recognized nuclear localization signal are the unique features of the nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19, which seems to have a little homology with the proteins of other members of this large corona virus family. These features also suggest the involvement of nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19 in different stages of viral lifecycle. The protein has multifaceted activities including packing of the nCoV/SARS-CoV-2/COVID-19 viral genome into a helical ribonucleocapsid (RNP) and playing an important role in viral self-assembly causing nCoV/SARS-CoV-2/COVID-19. The nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19 also forms dimer in the cell by self-association with the help of interactive C terminal domain.

Product Specifications

Host

Rabbit

Immunogen

SARS-CoV-2/COVID-19 Nucleocapsid (Ser2-Ala419) with His-tag in N-terminal was used as the immunogen for this antibody.

Target Antigen

Nucleocapsid

Clonality

Polyclonal

Applications

ELISA, WB

Purification

Protein A Chromatography

Format

Purified

Components

25 μg in 50 μl/100 μg in 200 μl PBS containing 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Recommended dilutions: WB: 1-2 μg/mL. ELISA : 2 μg/mL. However, this need to be optimized based on the research applications.

Isotype

Rabbit IgG

Conjugate

Biotin

Curated Selection

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