Polyclonal Antibody to 14-3-3
YWHAQ, also known as 14-3-3 tau (14-3-3tau) is a a phosphoserine-binding protein and a member of the 14-3-3 family of proteins. The 14-3-3 family members are dimeric phosphoserine/ phosphothreonine-binding proteins, which are involved in a very diverse spectrum of signaling pathways. YWHAQ plays a critical role in the regulation of E2F1 stability. It binds to Ser31-phosphorylated E2F1 and inhibits the ubiquitination of E2F1 during DNA damage. The binding of YWHAQ to E2F1 interferes with the function of an E31 ligase and therefore inhibits the ubiquitination and degradation of E2F1. This interaction is required for the expression and induction of several E2F1 apoptotic target genes as well as apoptosis during DNA damage.
Product Specifications
Product Name Alternative
14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1, YWHAQ
Gene Name
YWHAQ
Gene ID
10971
UniProt
P27348
Host
Rabbit
Reactivity
Human
Immunogen
A partial length recombinant 14-3-3 protein (amino acids 20-225) was used as the immunogen for this antibody.
Target Antigen
14-3-3
Clonality
Polyclonal
Applications
WB
Purification
Protein A Chromatography
Format
Purified
Components
25 μg in 50 μl/100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Western blot analysis: 1-2 μg/mL
Isotype
Rabbit IgG
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