Protein A Monoclonal Antibody (Clone: ABM4B3.1G3)
Protein A (PA) is a 42 kDa surface protein originally found in the cell wall of the Gram positive bacteria Staphylococcus aureus. It has the specific ability to bind with the constant (Fc) portion of immunoglobulin molecules from various different species. Protein A is encoded by the polymorphic X-region and functions as an immunoglobulin-binding protein, characteristically it has the ability to bind with the –Fc-region– of IgG. Due to its high affinity for Fc region of human IgG, it is extensively used in affinity chromatography process required for the purification of antibodies. However, one of the major disadvantages of this method of purification is co elution or leaching of PA along with the target antibody.
Product Specifications
Gene Name
Spa
Host
Mouse
Immunogen
Protein A from Staphylococcus aureus
Target Antigen
Protein A
Clonality
Monoclonal
Clone
ABM4B3.1G3
Applications
ELISA, WB
Purification
Protein G Chromatography
Format
Purified
Components
25 μg in 50 μl/100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Recommended dilutions: WB: 0.1-1 μg/mL, ELISA: 1 μg/mL. However, this need to be optimized based on the research applications.
Isotype
Mouse IgG2b, kappa
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
