Monoclonal Antibody to GAPDH (Clone: ABM22C5)
GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) is an enzyme best known for its role in glycolysis. However, extra-glycolytic functions of GAPDH have been described, including regulation of protein expression via RNA binding. GAPDH binds to numerous AREs (adenine-uridine rich elements) from various mRNA 3'-untranslated regions in vitro and in vivo despite its lack of a canonical RNA binding motif. GAPDH specifically catalyzes the simultaneous phosphorylation and oxidation of glyceraldehyde 3-phosphate using NAD+ (Nicotinamide Adenine Dinucleotide) as a cofactor to produce glycerate 1,3-biphosphate and NADH. In addition to its role in energy production, GAPDH has been implicated in many cellular processes including DNA repair tRNA export, membrane fusion and transport, endocytosis and nuclear membrane assembly, and cell death.
Product Specifications
Product Name Alternative
GAPDH||GAPD||CDABP0047||OK/SW-cl.12
Gene Name
GAPDH
Gene ID
2597
UniProt
P04406
Host
Mouse
Reactivity
Mouse, Human
Immunogen
A partial length recombinant GAPDH protein (amino acids 120-320) was used as the immunogen for this antibody.
Target Antigen
GAPDH
Clonality
Monoclonal
Clone
ABM22C5
Applications
IHC, WB
Purification
Protein G Chromatography
Format
Purified
Components
25 μg in 50 μl/100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Western blot analysis: 0.5-2 μg/mL, Immunohistochemical analysis: 5 μg/mL
Isotype
Mouse IgG1 Kappa
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