Mouse Monoclonal Antibody To N-Cadherin (Clone: 8C11)

N-cadherin (CD325) is a 140 kD protein belongs to a transmembrane molecules that mediate calcium dependent intracellular adhesion. Its extracellular region consists of five EC domains and has one cytoplasmic domain. N-cadherin is involved in organogenesis and maintenance of organ architecture by contributing to the sorting of heterogeneous cell types and in the cell adhesion needed to form tissues. N-cadherin is expressed by stem cells, myeloblasts, endothelial cells, and fibroblasts, and also is expressed in neural and muscle tissues and some types of carcinoma cells. CD325 associates with the cytoskeleton trough catenin proteins.

Product Specifications

Gene Name

CDH2

Gene ID

1000

UniProt

P19022

Host

Mouse

Reactivity

Mouse, Human

Immunogen

Recombinant human N-cadherin extracellular domain (exact sequence is proprietary)

Target Antigen

N-Cadherin

Clonality

Monoclonal

Clone

8C11

Applications

IHC, FACS, WB

Purification

Affinity Chromatography

Format

Purified

Components

0.5 mg/mL of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Flow Cytometry: 0.5-1 μg/10^6 cells, Immunofluorescence :1-2 μg/mL, Western Blotting: 0.5-1.0 μg/mL, Immunohistology (Formalin-fixed) : 0.5-1.0 μg/mL; for 30 minutes at RT. (Optimal dilution for a specific application should be determined.)