Monoclonal antibody to EpCAM (Clone: ABM4D85)
EpCAM is a Mr 40,000, type I transmembrane glycoprotein that consists of two epidermal growth factor-like extracellular domains, a cysteine-poor region, a transmembrane domain, and a short cytoplasmic tail. EpCAM is encoded by the GA733-2 gene located on the long arm of chromosome 4. EpCAM has been described by various names, including those associated with monoclonal antibodies specific for the cell surface antigen (MH99, AUA1, MOC31, 323/A3, KS1/4, GA733, and HEA125) and cDNA clones used to define the antigen KS 1/4, EGP, EGP40, and GA733-2.
Product Specifications
Product Name Alternative
EPCAM||GA733-2||M1S2||M4S1||MIC18||TACSTD1||TROP1
Gene Name
EPCAM
Gene ID
4072
UniProt
P16422
Host
Mouse
Reactivity
Human
Immunogen
A partial length recombinant human EpCAM protein (amino acids 11-236) was used as the immunogen for this antibody.
Target Antigen
EpCAM
Clonality
Monoclonal
Clone
ABM4D85
Applications
FACS, WB
Purification
Protein G Chromatography
Format
Purified
Components
25 μg in 50 μl/100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage Conditions
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Western blot analysis: 0.01-0.02 μg/mL, Flowcytometric analysis: 0.5-1 μg/10^6 cells
Isotype
Mouse IgG2a Kappa
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