Monoclonal Antibody to Kappa (Clone: ABM3A47)
The kappa light chain antibody recognizes the kappa light chain of immunoglobulin. The kappa light chain itself is one of the two small polypeptide subunits of an antibody, the other being lambda. Antibodies are produced by the B-cells in lymphoid tissue. Each B lymphocyte expresses either lambda or kappa light chain but never both together. Hence, the kappa light chain antibody is a useful marker for identifying B lymphocytes expressing kappa light chain.
Product Specifications
Product Name Alternative
IGKV1D-16
Gene Name
IGKV1D-16
Gene ID
28901
UniProt
P01601
Host
Mouse
Reactivity
Human
Immunogen
B lymphoma cells are used as immunogen for this antibody.
Target Antigen
Kappa
Clonality
Monoclonal
Clone
ABM3A47
Applications
IHC, FACS, WB
Purification
Protein G Chromatography
Format
Purified
Components
25 μg in 50 μl/100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage Conditions
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Immunohistochemical analysis: 1:400-1:800, Western blot analysis: 0.1-0.5 μg/mL, Flowcytometric analysis: 0.5-1 μg/10^6 cells
Isotype
Mouse IgG1 Kappa
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