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Monoclonal Antibody to EpCAM (CD326) Cytoplasmic Domain (Clone: ABM2C92)

EpCAM is a Mr 40,000, type I transmembrane glycoprotein that consists of two epidermal growth factor-like extracellular domains, a cysteine-poor region, a transmembrane domain, and a short cytoplasmic tail. EpCAM is encoded by the GA733-2 gene located on the long arm of chromosome 4. EpCAM has been described by various names, including those associated with monoclonal antibodies specific for the cell surface antigen (MH99, AUA1, MOC31, 323/A3, KS1/4, GA733, and HEA125) and cDNA clones used to define the antigen KS 1/4, EGP, EGP40, and GA733-2.

Product Specifications

Product Name Alternative

Epcam||Tacstd1

Gene Name

EPCAM

Gene ID

4072

UniProt

P16422

Host

Mouse

Reactivity

Human

Immunogen

A partial length recombinant human EpCAM (amino acids 110-314) was used as the immunogen for this antibody.

Target Antigen

EpCAM

Clonality

Monoclonal

Clone

ABM2C92

Applications

IHC, WB

Purification

Protein G Chromatography

Format

Purified

Components

25 μg in 50 μl/100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Western blot analysis: 4-6 μg/mL, Immunohistochemical analysis: 5 μg/mL

Isotype

Mouse IgG2b Kappa

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