Monoclonal Antibody to MBD2/MBD3 (Clone: ABM14A8)
MBD2 is a subunit of the NuRD complex that is postulated to mediate gene repression via recruitment of the complex to methylated DNA. This protein binds around 1 kb downstream of the transcription start site of a subset of 400 CpG island promoters that are characterized by the presence of active histone marks, RNA polymerase II (Pol2) and low to medium gene expression levels and H3K36me3 deposition. Methylated MBD2 is involved in silencing methylated tumor suppressor genes as well as activation of pro-metastatic genes. MBD2 also plays a key role in regulating expression of a range of genes that are associated with optimal DC (Dendritic cells) activation and function.
Product Specifications
Product Name Alternative
MBD3
Gene Name
MBD3
Gene ID
53615
UniProt
O95983
Host
Mouse
Reactivity
Human
Immunogen
A partial length recombinant MBD3 protein (amino acids 1-290) was used as the immunogen for this antibody.
Target Antigen
MBD2/MBD3
Clonality
Monoclonal
Clone
ABM14A8
Applications
IHC, FACS, WB
Purification
Protein G Chromatography
Format
Purified
Components
25 μg in 50 μl/100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Western blot analysis: 2-4 μg/mL, Immunohistochemical analysis: 5 μg/mL, FACS analysis: 0.5 μg/10^6 cells
Isotype
Mouse IgG1 Kappa
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