Monoclonal antibody to CD16 (Clone: B73.1)
CD16, also known as low affinity IgG receptor III (FcgammaRIII), is expressed as two distinct forms, referred to as CD16a and CD16b. CD16a (FcgammaRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein expressed on the surface of NK cells, activated monocytes, macrophages, a subset of T cells, and placental trophoblasts in humans. CD16b (FcgammaRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI) -anchored protein whose extracellular domain is over 95% homologous to that of CD16a, and is expressed specifically on neutrophils. CD16 binds to aggregated IgG or IgG-antigen complex, which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC) .
Product Specifications
Product Name Alternative
CD16-II, CD16a antigen, Fc-gamma RIII-alpha, FcR-10, IgG Fc receptor III-2, CD16A, FCG3, FCGR3, IGFR3
Gene Name
FCGR3A
Gene ID
2214
UniProt
P08637
Host
Mouse
Reactivity
Mouse, Human
Immunogen
NK cell-enriched fraction from human peripheral blood was used as the immunogen for this antibody.
Target Antigen
CD16
Clonality
Monoclonal
Clone
B73.1
Applications
FACS, IF
Purification
Protein G Chromatography
Format
Purified
Components
25 μg in 50 μl/100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage Conditions
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
FACS, IF
Isotype
Mouse IgG1k
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