Monoclonal Antibody to mouse MHC Class II (I-A/I-E) (Clone: M5/114) -NALE
MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
Product Specifications
Host
Rat
Reactivity
Mouse
Immunogen
Activated C57BL/6 mouse spleen cells was used as Immunogen
Target Antigen
MHC Class II (I-A/I-E)
Clonality
Monoclonal
Clone
M5/114
Applications
Functional Assay, IHC, FACS, WB, IF
Purification
Protein G
Format
Low Endotoxin, Azide Free
Components
PBS, pH 7.0 Contains no stabilizers or preservatives
Storage Conditions
The antibody solution should be stored undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.
Applications Notes
<2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay Functional assays, Immunofluorescence, Western blot, Immunoprecipitation, Flow cytometry
Isotype
Rat IgG2b, k
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