Monoclonal Antibody to Rat NKp46 (Clone: WEN23)

Rat NKp46 is an activating receptor expressed on all NK cells in all rat strains tested. NKp46 is the defining surface marker for rat NK cells. No expression of NKp46 has so far been reported on non-NK cells in the rat. NKp46 associates with the activating adaptor proteins FcepsilonRIgamma and CD3zeta and activates NK cell cytotoxicity and IFNgamma production.

Product Specifications

Product Name Alternative

Natural cytotoxicity triggering receptor 1, Activating receptor 1, Lymphocyte antigen 94 homolog, NK receptor KILR-1, Natural killer cell p46-related protein, CD335

Gene Name

Ncr1

Gene ID

117547

UniProt

Q9Z0H5

Host

Mouse

Reactivity

Rat

Immunogen

Recombinant rat NKp46 ectodomain coupled to the Fc portion of mouse IgG2b was used as the immunogen for this antibody.

Target Antigen

NKp46

Clonality

Monoclonal

Clone

WEN23

Applications

Functional Assay, IP, IHC, FACS, WB

Purification

Protein G Chromatography

Format

Purified

Components

25 μg in 50 μl/100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles

Applications Notes

Facs anaysis: 1-2 μg/10^6 Cells, Western blot analysis: 1-2 μg/mL, Immunoprecipitation: 1-2 μg/mL Reference: Ingunn H. Westgaard, Siri F. Berg, John T. Vaage, Lawrence L. Wang, Wayne M. Yokoyama, Erik Dissen, and Sigbjrn Fossum , Rat NKp46 activates natural killer cell cytotoxicity and is associated with Fc_x0001_RI and CD3, September 8, 2004 as DOI:10.1189/jlb.0903428