Monoclonal Antibody to DDX3 (Clone: ABM27H2)
DDX3 (DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked) is having a large family of ATP-dependent RNA helicases that involve in several steps of RNA metabolism. Main functions of DDX3 involve transcription, mRNA export and translation, regulation of apoptosis and hepatitis C virus (HCV) replication. DDX3 has two isoforms produced by alternative splicing. The cytoplasmic form is highly phosphorylated in the G1/S. DDX3 is primarily expressed in nucleus during G0 phase and in the cytoplasm during G1/S phase.
Product Specifications
Product Name Alternative
DDX3X||DBX||DDX3
Gene Name
DDX3X
Gene ID
1654
UniProt
O00571
Host
Mouse
Reactivity
Human, Mouse
Immunogen
A partial length recombinant DDX3 protein (amino acids 10-230) was used as the immunogen for this antibody.
Target Antigen
DDX3
Clonality
Monoclonal
Clone
ABM27H2
Applications
WB
Purification
Protein G Chromatography
Format
Purified
Components
25 μg in 50 μl/100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Western blot analysis: 2-4μg/mL
Isotype
Mouse IgG2b Kappa
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