Monoclonal Antibody to Bcl-2 (Clone: BC1)
Bcl-2 (B-cell lymphoma 2), is a member of Bcl-2 family of regulator proteins. These proteins contain a hydrophobic cleft that binds to BH3-only proteins and to the pro-apoptotic Bcl-2 family members Bad, Bak, and Bax to inhibit apoptosis. In the absence of this binding, the proapoptotic Bcl-2 members are recruited to the OMM (Outer Mitochondrial Membrane) at which they oligomerize and cause OMM permeabilization, releasing proapoptotic effectors such as SMAC or cytochrome-c. Bcl-2 also neutralize a group of 'sensor' proteins (such as BIM), which are triggered by cytotoxic stimuli such as chemotherapy. BCL-2 proteins therefore have a central role as guardians against apoptosis, helping cancer cells to evade cell death.
Product Specifications
Product Name Alternative
BCL2
Gene Name
BCL2
Gene ID
596
UniProt
P10415
Host
Mouse
Reactivity
Human
Immunogen
A synthetic peptide corresponding to amino acids 41-54 (GAAPAPGIFSSQPG-Cys), of human Bcl-2 was used as immunogen.
Target Antigen
Bcl-2
Clonality
Monoclonal
Clone
BC1
Applications
IHC, FACS, WB
Purification
Protein G Chromatography
Format
Purified
Components
25 μg in 50 μl/100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Western blot analysis: 2-4 μg/mL, Immunohistochemical analysis: 5 μg/mL, Flowcytometric analysis- 2-4 μg/10^6 Cells
Isotype
Mouse IgG1 Kappa
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