T7 RNA Polymerase, 100 U/μL, 50,000 U
Wild-type bacteriophage T7 RNA polymerase is a highly processive, 100 kDa, DNA-dependent RNA polymerase that catalyzes thein vitrotranscription (IVT) of a recombinant gene regulated by the T7 promoter. It is a key enzyme used to synthesize RNA from a DNA template. It is highly efficient and specific, capable of transcribing large quantities of RNA in a relatively short time frame. Its high robustness and reproducibility make it the standard industry choice in IVT as well as other applications such as radiolabeled RNA probe preparation and RNA construct development for additional studies. E-0053-001, -01, and -08 are provided at a 2X higher concentration than E-0053-S and -L for specialized applications and CleanCap® co-transcriptional capping of RNA constructs. Unit definition: One unit of enzyme incorporates 1 nmol of ATP in 1 hour at 37°C.
Product Specifications
Volume
0.05 mL, 0.1 mL, 0.5 mL, 4.2 mL
Host
E. coli
Reaction Volume
25
Concentration
50 U/μL, 100 U/μL
Buffer
50mM Tris, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 50% glycerol, 0.1% Tween-20, pH 8.0
Storage Conditions
-20 to -25°C
Applications Notes
Frequently Asked Questions
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