Inorganic Pyrophosphatase (iPPase), 12 U

E. coli inorganic pyrophosphatase (iPPase) catalyzes the hydrolysis of inorganic pyrophosphate (PPi) to form orthophosphate. Using iPPase in anin vitrotranscription reaction drives RNA synthesis forward by removing pyrophosphate which forms as a byproduct and precipitates with key transcription buffer cations. Unit definition: One unit of enzyme produces 1 μmol of phosphate from inorganic pyrophosphate in 1 min at 25°C.

Product Specifications

Volume

60 μL, 630 μL

Host

E. coli

Reaction Volume

Concentration

0.2 U/μL

Buffer

20 mM Tris, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, pH 8.0

Storage Conditions

-20 to -25°C

Applications Notes

Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission from TriLink No license under any patent or other intellectual property right of TriLink or its licensors is granted or implied by the purchase unless otherwise provided in writing.