CleanScribe RNA Polymerase, 100 U/μL, 420,000 U

TriLink’s CleanScribeTM RNA Polymerase is a novel DNA-dependent RNA polymerase that catalyzes the in vitro transcription (IVT) of a recombinant gene regulated by the T7 promoter. This enzyme reduces double-strand RNA (dsRNA) levels by up to 85% during IVT. dsRNA is a byproduct of IVT reactions and can trigger undesirable inflammatory responses in host cells. This novel RNA polymerase can directly substitute a wild-type T7 RNA polymerase in an IVT protocol to synthesize RNAs from a DNA template. It is highly efficient and specific, capable of transcribing large quantities of RNA in a relatively short time frame. Its robustness, reproducibility, and dsRNA reduction make it ideal for RNA synthesis as well as other applications such as radiolabeled RNA probe preparation and RNA construct development for additional studies. E-0107-001, -01, and -08 are provided at a 2X higher concentration than E-0107-S and -L for specialized applications and CleanCap® co-transcriptional capping of RNA constructs following TriLink’s protocols. E-0107-10 is supplied at a 10X higher concentration than E-0107-S and -L, and it includes 10 mL of dilution buffer to facilitate the optimization of reaction parameters. Unit definition: One unit of enzyme incorporates 1 nmol of ATP in 1 hour at 37°C.